Only a few steps are required to modify the genome with Cas9-triggered homologous recombination.
Expand the items below to view a protocol for each step, or download our Cloning Guide and Injection Protocols.
Expand the items below to view a protocol for each step, or download our Cloning Guide and Injection Protocols.
▸ Construct design and cloning
▸ Cas9-triggered homologous recombination with unc-119 selection
▾ Removal of the unc-119(+) cassette with Cre recombinase
These instructions assume you are using unc-119 selection, but the same basic approach should be applicable to other selectable markers.
o 2.5 ng/μL pCFJ90 (Pmyo-2::mCherry pharyngeal co-injection marker) or any other fluorescent marker.
|