LOCUS his_72_GFP_repai 10371 bp ds-DNA circular 12-DEC-2013 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT Note a unique feature of this particular construct: COMMENT Because his-72 and the downstream Y57B8A.33 gene are oriented on the same strand, the his-72 3'UTR sequence might be part of the Y57B8A.33 promoter. To avoid disrupting this promoter, we duplicated the his-72 3'UTR, with one copy immediately after the GFP and a second copy downstream of unc-119(+). One of the copies is located between the LoxP sites so that it will be removed along with the unc-119(+) cassette. COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers misc_recomb join(337..1895,1899..1909,2796..2798,2833..2875,2878..3095, 5521..7189) /label=Genomic Sequence /ApEinfo_fwdcolor=#cccccc /ApEinfo_revcolor=#cccccc /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_recomb join(5258..5300,5303..5470) /label=Genomic Sequence(1) /ApEinfo_label=Genomic Sequence /ApEinfo_fwdcolor=#cccccc /ApEinfo_revcolor=#cccccc /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_recomb 5471..5520 /label=Genomic Sequence(2) /ApEinfo_label=Genomic Sequence /ApEinfo_fwdcolor=#cccccc /ApEinfo_revcolor=#cccccc /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS join(1444..1725,1785..1910,2796..2798) /label=HIS-72 (a isoform) /ApEinfo_fwdcolor=#00cccc /ApEinfo_revcolor=#006666 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS join(4084..4140,4236..4351,4395..4771,4823..4929) /label=Cbri unc-119 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_recomb 5224..5257 /label=LoxP /ApEinfo_fwdcolor=steel blue /ApEinfo_revcolor=orange /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS join(5572..5623,6001..6146,6648..6768) /label=Y57B8A.33 /ApEinfo_fwdcolor=#00cccc /ApEinfo_revcolor=#006666 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 337..356 /label=his-72 Homology L #215 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=#00cc99 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(7171..7189) /label=his-72 Homology R #216 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=#00cc99 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 3'UTR join(2833..2875,2878..3045) /label=his-72 3'UTR /ApEinfo_fwdcolor=#cc3333 /ApEinfo_revcolor=#cc3333 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 3'UTR join(5258..5300,5303..5470) /label=his-72 3'UTR(1) /ApEinfo_label=his-72 3'UTR /ApEinfo_fwdcolor=#cc3333 /ApEinfo_revcolor=#cc3333 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS join(1929..2099,2151..2304,2356..2519,2571..2795) /label=GFP /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 promoter 3096..4083 /label=unc-119 Promoter /ApEinfo_fwdcolor=#33cc00 /ApEinfo_revcolor=#33cc00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 3'UTR 4930..5223 /label=unc-119 terminator /ApEinfo_fwdcolor=#cc3333 /ApEinfo_revcolor=#cc3333 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(3211..3230) /label=unc119 5' out /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=#00cc99 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 3317..3787 /translation="MLKLTQIKLHDKFIALFNSSWLIICYSVPILSAPYTPLHTLSSF LSWIVSSSPQLCYFYDLRPLLFRSFLSSLTSSSASFLLPFSLLNLPLFPISVNHSKKK KKKTLIHFIFFSVGVCCSVKSELSIPRLLLFLLRFESITPPKRIRFWDSPRGSQ*" /label=ORF frame 1 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 5095..5114 /label=unc119 3' out /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_recomb 2799..2832 /label=LoxP(1) /ApEinfo_label=LoxP /ApEinfo_fwdcolor=steel blue /ApEinfo_revcolor=orange /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_difference 5301..5302 /label=PAM Mutations to block Cas9 cleavage of repair template /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_difference 2876..2877 /label=PAM Mutations to block Cas9 cleavage of repair template(1) /ApEinfo_label=PAM Mutations to block Cas9 cleavage of repair template /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_signal complement(2879..2905) /label=Cas9 target sequence /ApEinfo_fwdcolor=#ff6600 /ApEinfo_revcolor=#ff6600 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_signal complement(5304..5330) /label=Cas9 target sequence(1) /ApEinfo_label=Cas9 target sequence /ApEinfo_fwdcolor=#ff6600 /ApEinfo_revcolor=#ff6600 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC 61 ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC 121 TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA 181 TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCTAT 241 TTAGGTGACG CGTTAGAATA CTCAAGCTAT GCATCAAGCT TGGTACCGAG CTCGGATCCA 301 CTAGTAACGG CCGCCAGTGT GCTGGAATTC GCCCTTgacc ccacaaaatc gatacgacaa 361 aaggggggag gaggcggaga cttggcacaa tgccaagtct ttgctctctg tgtcgtcacg 421 tgtcttcgtg ttggctgagt atcaaacgtt atagtgtgga caccaatttg gcttcgtact 481 tcttactcgt tcccgccccg ccccgacacc attcgaatat atattgtcag ttgttctgtt 541 tgtcgtcgtg atgagaggtg cgtgcgtgtg cgcgcgcgct ctcccaactt ttcggaccat 601 tttgcgggct ttatactgca atttggctca tttcttctcc ttttttttcc acaggcttat 661 tttgtactct cgagttgaca gcctttttcg gtataggttg gaagtagtcc attaaagtag 721 atgtttctta agaatttatc ggcttagcat cgatttcgac ctagttagat gccacgttta 781 tgcgcgaagc ggccgcgtcg cggcgagaat ttcagtgcgg ttttacggga ttatccatta 841 aaatcaggaa aatcagcaga aaacttctta acaaaactaa tttttttaga aaagaattta 901 gaaatacgta gtcttgaata attcagcaac tttttcaaat tttatgagaa tttcgcaatt 961 tcctacacct cctatttcat catcacttcc gaaatcctac gttatcgatc gaacacgcga 1021 cgcgaccgca gcgcacctac taagcgcgcg catttcgacc gtgacgcgac cgcaacgcgg 1081 tggtgtcagg gtgcgcgcgc tttttcaatc atatgtggtg cggtcgattc cttcattcat 1141 ccttctgtat gcagctgctc atcagtttat tgagcatctg ctgtaccaat tcgtacttta 1201 aattaaatat taagagtaca ttttctgtgt gctctgtata taaatgtcta gaataaactg 1261 caaacaagaa ttcttggctc catctttccc tgaatcaata tcgatcgata gtgacacgcg 1321 acgcgaccgc aacgcgtagg gccatttcat attgattctt cttctactct tccttcttct 1381 ccttctgctc tcacatttcc tcactattcc aaccattctc aattctcaat ttccagaaca 1441 acaATGGCTC GTACCAAGCA AACCGCTCGT AAATCCACCG GAGGAAAGGC TCCAAGAAAG 1501 CAGTTGGCCA CCAAGGCCGC CCGCAAATCG GCTCCAACCA CCGGAGGAGT CAAGAAGCCA 1561 CATCGTTACC GTCCAGGAAC TGTCGCTCTC CGTGAGATTC GTCGTTACCA GAAGTCGACT 1621 GAGCTTCTCA TCCGCAAGCT TCCTTTCCAA CGTCTCGTTC GTGAGATTGC CCAGGACTTC 1681 AAGACTGATC TCCGCTTCCA GTCGGCTGCC ATCGGAGCTC TCCAGgtttg tagtttgaat 1741 ttgaaattta aaaaaaatta attaaaatct tttttttttt ccagGAGGCA TCTGAAGCAT 1801 ACCTCGTCGG ACTCTTCGAG GACACCAACC TGTGCGCCAT CCACGCCAAG CGCGTCACCA 1861 TCATGCCAAA GGACATGCAA CTCGCCAGAC GCATCagaGG AGAACGTGCc tcaggagcta 1921 gcggagccAT GAGTAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT CTTGTTGAAT 1981 TAGATGGTGA TGTTAATGGG CACAAATTTT CTGTCAGTGG AGAGGGTGAA GGTGATGCAA 2041 CATACGGAAA ACTTACCCTT AAATTTATTT GCACTACTGG AAAACTACCT GTTCCATGGG 2101 TAAGTTTAAA CATATATATA CTAACTAACC CTGATTATTT AAATTTTCAG CCAACACTTG 2161 TCACTACTTT CTGTTATGGT GTTCAATGCT TCTCGAGATA CCCAGATCAT ATGAAACGGC 2221 ATGACTTTTT CAAGAGTGCC ATGCCCGAAG GTTATGTACA GGAAAGAACT ATATTTTTCA 2281 AAGATGACGG GAACTACAAG ACACGTAAGT TTAAACAGTT CGGTACTAAC TAACCATACA 2341 TATTTAAATT TTCAGGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT AATAGAATCG 2401 AGTTAAAAGG TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA TTGGAATACA 2461 ACTATAACTC ACACAATGTA TACATCATGG CAGACAAACA AAAGAATGGA ATCAAAGTTG 2521 TAAGTTTAAA CATGATTTTA CTAACTAACT AATCTGATTT AAATTTTCAG AACTTCAAAA 2581 TTAGACACAA CATTGAAGAT GGAAGCGTTC AACTAGCAGA CCATTATCAA CAAAATACTC 2641 CAATTGGCGA TGGCCCTGTC CTTTTACCAG ACAACCATTA CCTGTCCACA CAATCTGCCC 2701 TTTCGAAAGA TCCCAACGAA AAGAGAGACC ACATGGTCCT TCTTGAGTTT GTAACAGCTG 2761 CTGGGATTAC ACATGGCATG GATGAACTAT ACAAATAAAT AACTTCGTAT AGCATACATT 2821 ATACGAAGTT ATgctccatc accaattctc gaagcacttt taatattcat ttaatAAaac 2881 ttgtaccata atcctcgtat ttttccatcc cgaaaattga tttttgcgat cgaaccgccg 2941 atctcatcac cacttttctc tccgttcttt atgtcttatt ttattcgtaa attgctgaat 3001 ttcatgttga actccaacaa aaaaaaataa agtttcgtgt gcttcagtag tctagcaaca 3061 tcatatgaac gcctagcaac tatattccac gccgcCTTTG AGCCAATTTA TCCAAGTCCT 3121 TGTAAAAAGT ATTCGAAAAT TGTTAACGGA TAAAATGTTT ATTATAATAT CAAAAACAAT 3181 TTGTCAGTTG ACCACTTTTT GATATATTTG ACAGAAACCG GGATGAATTG GCTCAAAGTT 3241 AGGGCGCCCT TCTATTACAG GGTTTCTGAT AACAAACCGG TTATTAACTC CCAACAAGGG 3301 ATGATTTCAA TTCATCATGC TCAAATTGAC CCAAATTAAG TTACATGACA AATTCATCGC 3361 CCTTTTCAAC TCTTCTTGGC TCATCATCTG TTATTCTGTT CCTATTCTGT CTGCACCCTA 3421 TACCCCTTTG CATACTCTCT CGTCATTCCT TTCTTGGATA GTGTCTTCTT CTCCCCAGCT 3481 CTGCTACTTC TATGACTTGC GCCCGCTGCT TTTCCGCTCG TTCCTCTCCT CTCTGACGTC 3541 TTCTTCTGCT TCTTTTCTCC TTCCTTTTTC CCTTCTCAAT CTTCCTCTTT TTCCCATTTC 3601 TGTCAATCAT TCGAAGAAGA AGAAGAAGAA GACCCTCATT CATTTCATTT TTTTTTCTGT 3661 CGGTGTGTGC TGCTCGGTTA AGAGTGAGCT CTCTATTCCA CGTCTTCTTC TTTTTCTTCT 3721 TCGATTCGAA TCAATCACTC CACCAAAACG CATTCGTTTT TGGGATTCAC CCCGCGGTTC 3781 GCAATAGGTT TCTTTTTCAA ATTATTAGCG TTATAAATAG AAAAATGGTG GAGTTTCAAT 3841 AAAAATGATA ATTTACAAAG TGTATTTTGA TTACATGTAC TCAAAAAGGT TGAAATTTTT 3901 CATACCAGTT TTCCGGAAAT CCATCTGATA TCATTATCGT ATTTTTCTTT TTAAAAATGT 3961 TTTCAAAAAA AAACAAAATA TAGCTGGTTA TTTGGCACCC TCTAATTACC ATTTTCCTGT 4021 CACACCACAC TCTTTTCCTT CTTCCACTTC TTTCCGTTTT CAGCCGCTTC CAACCAAACC 4081 GATATGAAAG CCGAGCAACA ACGATCGATT CCACCCGGCT CGGCGACCTT CCCGTCGCAG 4141 GTGAGACTCA GAAAACTAGA GAAACCGCTC AACTAACTCT TGATATCCGA TTTCATTCTT 4201 TTCTCTTTTC TTTTTGTTGA ACTTTTCCAT ATTTCCAGAT GCCACGGCCA CCACCAAGCA 4261 CCGAACAAGG AATCACAACG GAATCGGAGC TTGCGAAGAA AGCTCAAATC ACTCCGAACG 4321 ACGTTTTAGC ACTTCCGGGA ATCACTCAAG GTATCTTGCT CCCTTTGGAT TTCAGTTTAA 4381 AACATAAATT TAGGATTCTT ATGCTCCCCA TCTGCGAACA TCTATAACAT CGAGTTCACC 4441 AAGTTCCAAA TCCGTGATCT GGACACTGAG CAAGTGCTTT TCGAGATCGC CAAACCGGAG 4501 AACGATCAGG AGAATGATGA GTCGCCACAG GAGTCGGCAA GATACGTGCG TTATAGATTT 4561 GCTCCAAACT TTTTGAAACT CAAAACGGTC GGAGCAACTG TGGAATTCAA AGTAGGAGAC 4621 ATCCCAATCC ATCATTTCCG AATGATCGAA CGTCACTTCT TCAAAGATCG CCTTCTGAAG 4681 TGTTTTGACT TTGAATTCGG ATTCTGTATT CCGAATTCAC GAAACAACTG TGAACATATC 4741 TATGAGTTCC CTCAACTCTC TCAACAACTC AGTGAGTCAT TATTCTAAAA AGTACAATTC 4801 AAAAGACTAA TCTCTTTTTC AGTGGACGAC ATGATCAACA ATCCAAACGA GACTCGTTCT 4861 GACAGCTTCT ATTTCGTCGA TAACAAACTC GTCATGCACA ACAAAGCCGA CTACTCATAT 4921 GATGCATAAA TATTTAATAC AAAAATGTTC TGGATAATTA TTCTGTCGAA TAGAAAAAAA 4981 ACTCCAAATG TGATTAAATT CCAATAATTC CTGTCTAGTT TGTTCCTTCC CCTTCCCCTT 5041 CTCATGTTCA ATGCATTCCT AAGCTTTTCA GTTCCCCCCT TGTTTCTATA TTTTTTCGCT 5101 GTCCTGTCAC ACTCGCTAAA AACACTAATC ACACGGAAAT CTGTTTTCAA TAAAAACTCC 5161 AACTTTAACT CATTTTCAAT TTCAACTGAA AGATTTTTTC ATTAGAGAAT GTCTAGAACT 5221 AGGATAACTT CGTATAGCAT ACATTATACG AAGTTATgct ccatcaccaa ttctcgaagc 5281 acttttaata ttcatttaat AAaacttgta ccataatcct cgtatttttc catcccgaaa 5341 attgattttt gcgatcgaac cgccgatctc atcaccactt ttctctccgt tctttatgtc 5401 ttattttatt cgtaaattgc tgaatttcat gttgaactcc aacaaaaaaa aataaagttt 5461 cgtgtgcttc agtagtctag caacatcata tgaacgccta gcaactatat tccacgccgc 5521 cgcagttttg aattgaattc actaaattca tgctctgaat tcacaaaaaa aATGTTTCTT 5581 CGAGTCATTG GAAATAGTAA AAAACGCCAG CGACTATTTA TCAgtaagtt ttaaaagggt 5641 ctcgtcgcga aattcacatt aaaaatatta attttctgta tgtaccttta aaattactgt 5701 agaaaatcta tcagatgggt cttggcgcgc aaaacgcaaa aaataaattg atttcattgc 5761 atgctttttc cgcgccgaga cccatctaac ctaaattttt caaaaaggtg tgcgccttta 5821 aagattactg tagtttcgaa cttgtgtagt tgcggagttt tcatcgattt ttttgtaaat 5881 aaatagtttt attttagttt ttgaagaaat aaatattttt tcaacgaaca actatatgaa 5941 aaatcgataa aaatcccgca attttttgga aaattctgtg atggataata ccaaattcag 6001 ATGTTTCCTT CTTTTTTCTC ATCATTGGGC TTCTTGTTCT CGGTGTCTTC ACAATCAAAA 6061 AGAACACCTA TATCACCTTC GATTCATGGG ATCTAGCACA AGAGATTGCT CAGAACGCGA 6121 CGGATGCAGC GATGGAAAAC TCTTCTgtaa gtttttcacg gaattctggc ctccctcata 6181 aatcgaaatg gcagagtttg ccgaactagg ccattttgag tccgcgagat tttgtgtagg 6241 tttacggcgc gttgcgtgtc gcgtcgcggc tcgtttttag ttgtaaaacc gatgtatttg 6301 tccgtgtgga gtacactaca cttttccacg cgttgtccgg caggcgattg ccaatggagc 6361 gcgaaaattc aatgagaaat gccagaaacc cgtgtttttt tgcacggatt tctggcttcc 6421 ctcataaatt gaaatggaag agttttttgc cgaactaggc caggccatat ctggggtaga 6481 tttacggcgc gttgcgtgtc gcgtcgcggt tcgattgtaa aacgaaatgc atttgtccgt 6541 gtggagtaca cgactttccc agggcgattg tcaatggagc gcgaacaatt caatgaggaa 6601 ggccagaagc ccgtgaattt gaaaaaaaaa ctaaatttca attatagCAC ATTGATTTCT 6661 CTACTGTTCT GTGGAATGCT GACATGGAAT TGGTTTTAGC GAATAAAACG ATTGATGGGC 6721 CTCCGAAAAG AGATATTCTA TTCGTTGTCA GTACTTTTAT CGGAGTTGgt aagttgaatt 6781 cgatatgcca tattattagt tttataaaat acttattacg gagacacaaa attctgaaaa 6841 tgcgtattgc gcaacgcatt ttacgctcaa aatatctcgt agcgaaaaga aaactacagt 6901 aattctttaa atgactactg tagcgcttgt gtcgatttac gggctccatc agcgatattc 6961 tattgtcttt gggttcttcg tattaatttt tttccatttc tttacctaat ttgaatgttc 7021 taaaaataaa taaaccgtaa atccacacta caatagtcat tgaaagaatt actgtagttt 7081 tcttttcgct acgagatatt ttgcgcgtca aatatgttgc acaatacgca ttcttaaaat 7141 ttttccggta atatgagccc atttacctgc ctcagagcaa attcagcctA AGGGCGAATT 7201 CTGCAGATAT CCATCACACT GGCGGCCGCT CGAGCATGCA TCTAGAGGGC CCAATTCGCC 7261 CTATAGTGAG TCGTATTACA ATTCACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA 7321 CCCTGGCGTT ACCCAACTTA ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA 7381 TAGCGAAGAG GCCCGCACCG ATCGCCCTTC CCAACAGTTG CGCAGCCTAT ACGTACGGCA 7441 GTTTAAGGTT TACACCTATA AAAGAGAGAG CCGTTATCGT CTGTTTGTGG ATGTACAGAG 7501 TGATATTATT GACACGCCGG GGCGACGGAT GGTGATCCCC CTGGCCAGTG CACGTCTGCT 7561 GTCAGATAAA GTCTCCCGTG AACTTTACCC GGTGGTGCAT ATCGGGGATG AAAGCTGGCG 7621 CATGATGACC ACCGATATGG CCAGTGTGCC GGTCTCCGTT ATCGGGGAAG AAGTGGCTGA 7681 TCTCAGCCAC CGCGAAAATG ACATCAAAAA CGCCATTAAC CTGATGTTCT GGGGAATATA 7741 AATGTCAGGC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTCA CGTAGAAAGC 7801 CAGTCCGCAG AAACGGTGCT GACCCCGGAT GAATGTCAGC TACTGGGCTA TCTGGACAAG 7861 GGAAAACGCA AGCGCAAAGA GAAAGCAGGT AGCTTGCAGT GGGCTTACAT GGCGATAGCT 7921 AGACTGGGCG GTTTTATGGA CAGCAAGCGA ACCGGAATTG CCAGCTGGGG CGCCCTCTGG 7981 TAAGGTTGGG AAGCCCTGCA AAGTAAACTG GATGGCTTTC TTGCCGCCAA GGATCTGATG 8041 GCGCAGGGGA TCAAGCTCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA 8101 AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG 8161 GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG 8221 CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AAGACGAGGC 8281 AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT 8341 CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC 8401 ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA 8461 TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC 8521 ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG 8581 GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGAGC ATGCCCGACG GCGAGGATCT 8641 CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC 8701 TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC 8761 TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA 8821 CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT 8881 CTGAATTATT AACGCTTACA ATTTCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG 8941 TATTTCACAC CGCATCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT 9001 TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC 9061 TTCAATAATA GCACGTGAGG AGGGCCACCA TGGCCAAGTT GACCAGTGCC GTTCCGGTGC 9121 TCACCGCGCG CGACGTCGCC GGAGCGGTCG AGTTCTGGAC CGACCGGCTC GGGTTCTCCC 9181 GGGACTTCGT GGAGGACGAC TTCGCCGGTG TGGTCCGGGA CGACGTGACC CTGTTCATCA 9241 GCGCGGTCCA GGACCAGGTG GTGCCGGACA ACACCCTGGC CTGGGTGTGG GTGCGCGGCC 9301 TGGACGAGCT GTACGCCGAG TGGTCGGAGG TCGTGTCCAC GAACTTCCGG GACGCCTCCG 9361 GGCCGGCCAT GACCGAGATC GGCGAGCAGC CGTGGGGGCG GGAGTTCGCC CTGCGCGACC 9421 CGGCCGGCAA CTGCGTGCAC TTCGTGGCCG AGGAGCAGGA CTGACACGTG CTAAAACTTC 9481 ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC 9541 CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT 9601 CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC 9661 CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT 9721 TCAGCAGAGC GCAGATACCA AATACTGTTC TTCTAGTGTA GCCGTAGTTA GGCCACCACT 9781 TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG 9841 CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA 9901 AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA 9961 CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG 10021 GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG 10081 AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC 10141 TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA 10201 ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG 10261 CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC 10321 GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA G //