Our papers on Cas9-triggered homologous recombination:
Dickinson DJ, Pani AM, Heppert JK, Higgins CD and Goldstein B (2015). Streamlined genome engineering with a self-excising drug selection cassette. Genetics Early Online. DOI: 10.1534/genetics.115.178335.
Dickinson DJ, Ward JD, Reiner DJ and Goldstein B (2013). Engineering the Caenorhabditis elegans genome using Cas9-triggered homologous recombination. Nature Methods 10:1028-1034. DOI: 10.1038/nmeth.2641.
Dickinson DJ, Pani AM, Heppert JK, Higgins CD and Goldstein B (2015). Streamlined genome engineering with a self-excising drug selection cassette. Genetics Early Online. DOI: 10.1534/genetics.115.178335.
Dickinson DJ, Ward JD, Reiner DJ and Goldstein B (2013). Engineering the Caenorhabditis elegans genome using Cas9-triggered homologous recombination. Nature Methods 10:1028-1034. DOI: 10.1038/nmeth.2641.
Resources for genome-editing techniques:
The Worm CRISPR Forum is a discussion group for users of the CRISPR/Cas9 system in C. elegans.
The CRISPR Design Tool from Feng Zhang's lab allows users to choose specific Cas9 target sites.
We use NEBuilder HiFi DNA Assembly Master Mix from NEB to build all of our homologous repair templates. This cloning approach allows multiple fragments to be assembled together seamlessly and in a single step.
Christian Frøkjær-Jensen's web site has a comprehensive overview of Mos1-based genome engineering strategies. Cas9-triggered homologous recombination relies on the same DNA repair pathway as several Mos1-based methods.
The Worm CRISPR Forum is a discussion group for users of the CRISPR/Cas9 system in C. elegans.
The CRISPR Design Tool from Feng Zhang's lab allows users to choose specific Cas9 target sites.
We use NEBuilder HiFi DNA Assembly Master Mix from NEB to build all of our homologous repair templates. This cloning approach allows multiple fragments to be assembled together seamlessly and in a single step.
Christian Frøkjær-Jensen's web site has a comprehensive overview of Mos1-based genome engineering strategies. Cas9-triggered homologous recombination relies on the same DNA repair pathway as several Mos1-based methods.
Other papers using Cas9 in C. elegans:
Paix A, Wang Y, Smith H, Lee CY, Calidas D, Lu T, Smith J, Schmidt H, Krause M and Seydoux G (2014). Scalable and versatile genome editing using linear DNAs with micro-homology to Cas9 sites in Caenorhabditis elegans. Genetics Advance Online Publication. DOI: 10.1534/genetics.114.170423
Arribere JA, Bell RT, Fu BX, Artiles KL, Hartman PS and Fire AZ (2014). Efficient marker-free recovery of custom genetic modifications with CRISPR/Cas9 in Caenorhabditis elegans. Genetics Advance Online Publication. DOI: 10.1534/genetics.114.169730.
Shen Z, Zhang X, Chai Y, Zhu Z, Yi P, Feng G, Li W and Ou G (2014). Conditional knockouts generated by engineered CRISPR-Cas9 endonuclease reveal the roles of coronin in C. elegans neural development. Developmental Cell 30:625-36. DOI: 10.1016/j.devcel.2014.07.017.
Kim H, Ishidate T, Ghanta KS, Seth M, Conte D Jr., Shirayama M and Mello CC (2014). A co-CRISPR strategy for efficient genome editing in Caenorhabditis elegans. Genetics 197:1069-80. DOI: 10.1534/genetics.114.166389v1.
Zhao P, Zhang Z, Ke H, Yue Y and Xue D (2014). Oligonucleotide-based targeted gene editing in C. elegans via the CRISPR/Cas9 system. Cell Research 24:247-50. DOI: 10.1038/cr.2014.9.
Chen C, Fenk LA and de Bono M (2013). Efficient genome editing in Caenorhabditis elegans by CRISPR-targeted homologous recombination. Nucleic Acids Research 41:e193. DOI: 10.1093/nar/gkt805.
Chiu H, Schwartz HT, Antoshechkin I, Sternberg PW (2013). Transgene-Free Genome Editing in Caenorhabditis elegans Using CRISPR-Cas. Genetics 195:1167-1171. DOI: 10.1534/genetics.113.155879.
Cho S. W., Lee J., Carroll D., Kim J.-S., Lee J (2013). Heritable gene knockout in C. elegans by direct injection of Cas9-sgRNA ribonucleoproteins. Genetics 195:1177-1180. DOI: 10.1534/genetics.113.155853.
Katic I, Großhans H (2013). Targeted Heritable Mutation and Gene Conversion by Cas9-CRISPR in Caenorhabditis elegans. Genetics 195:1173-1176. DOI: 10.1534/genetics.113.155754.
Lo TW, Pickle CS, Lin S, Ralston EJ, Gurling M, Schartner CM, Bian Q, Doudna JA, Meyer BJ (2013). Precise and Heritable Genome Editing in Evolutionarily Diverse Nematodes Using TALENs and CRISPR/Cas9 to Engineer Insertions and Deletions. Genetics 195:331-348. DOI: 10.1534/genetics.113.155382.
Tzur YB, Friedland AE, Nadarajan S, Church GM, Calarco JA and Colaiacovo MP. Heritable custom genomic modifications in Caenorhabditis elegans via a CRISPR-Cas9 system. Genetics 195:1181-1185. DOI: 10.1534/genetics.113.156075.
Waaijers S, Portegijs V, Kerver J, Lemmens BB, Tijsterman M, van den Heuvel S and Boxem M (2013). CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans. Genetics 195:1187-1191. DOI: 10.1534/genetics.113.156299.
Friedland AE, Tzur YB, Esvelt KM, Colaiacovo MP, Church GM and Calarco JA (2013). Heritable genome editing in C. elegans via a CRISPR-Cas9 system. Nature Methods 10:741-743.
Paix A, Wang Y, Smith H, Lee CY, Calidas D, Lu T, Smith J, Schmidt H, Krause M and Seydoux G (2014). Scalable and versatile genome editing using linear DNAs with micro-homology to Cas9 sites in Caenorhabditis elegans. Genetics Advance Online Publication. DOI: 10.1534/genetics.114.170423
Arribere JA, Bell RT, Fu BX, Artiles KL, Hartman PS and Fire AZ (2014). Efficient marker-free recovery of custom genetic modifications with CRISPR/Cas9 in Caenorhabditis elegans. Genetics Advance Online Publication. DOI: 10.1534/genetics.114.169730.
Shen Z, Zhang X, Chai Y, Zhu Z, Yi P, Feng G, Li W and Ou G (2014). Conditional knockouts generated by engineered CRISPR-Cas9 endonuclease reveal the roles of coronin in C. elegans neural development. Developmental Cell 30:625-36. DOI: 10.1016/j.devcel.2014.07.017.
Kim H, Ishidate T, Ghanta KS, Seth M, Conte D Jr., Shirayama M and Mello CC (2014). A co-CRISPR strategy for efficient genome editing in Caenorhabditis elegans. Genetics 197:1069-80. DOI: 10.1534/genetics.114.166389v1.
Zhao P, Zhang Z, Ke H, Yue Y and Xue D (2014). Oligonucleotide-based targeted gene editing in C. elegans via the CRISPR/Cas9 system. Cell Research 24:247-50. DOI: 10.1038/cr.2014.9.
Chen C, Fenk LA and de Bono M (2013). Efficient genome editing in Caenorhabditis elegans by CRISPR-targeted homologous recombination. Nucleic Acids Research 41:e193. DOI: 10.1093/nar/gkt805.
Chiu H, Schwartz HT, Antoshechkin I, Sternberg PW (2013). Transgene-Free Genome Editing in Caenorhabditis elegans Using CRISPR-Cas. Genetics 195:1167-1171. DOI: 10.1534/genetics.113.155879.
Cho S. W., Lee J., Carroll D., Kim J.-S., Lee J (2013). Heritable gene knockout in C. elegans by direct injection of Cas9-sgRNA ribonucleoproteins. Genetics 195:1177-1180. DOI: 10.1534/genetics.113.155853.
Katic I, Großhans H (2013). Targeted Heritable Mutation and Gene Conversion by Cas9-CRISPR in Caenorhabditis elegans. Genetics 195:1173-1176. DOI: 10.1534/genetics.113.155754.
Lo TW, Pickle CS, Lin S, Ralston EJ, Gurling M, Schartner CM, Bian Q, Doudna JA, Meyer BJ (2013). Precise and Heritable Genome Editing in Evolutionarily Diverse Nematodes Using TALENs and CRISPR/Cas9 to Engineer Insertions and Deletions. Genetics 195:331-348. DOI: 10.1534/genetics.113.155382.
Tzur YB, Friedland AE, Nadarajan S, Church GM, Calarco JA and Colaiacovo MP. Heritable custom genomic modifications in Caenorhabditis elegans via a CRISPR-Cas9 system. Genetics 195:1181-1185. DOI: 10.1534/genetics.113.156075.
Waaijers S, Portegijs V, Kerver J, Lemmens BB, Tijsterman M, van den Heuvel S and Boxem M (2013). CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans. Genetics 195:1187-1191. DOI: 10.1534/genetics.113.156299.
Friedland AE, Tzur YB, Esvelt KM, Colaiacovo MP, Church GM and Calarco JA (2013). Heritable genome editing in C. elegans via a CRISPR-Cas9 system. Nature Methods 10:741-743.