Only a few steps are required to modify the genome with Cas9-triggered homologous recombination. All of the protocols you need are right here.
Protocol for constructing repair templates with SapTrap assembly.
Homology arm template files that go with this protocol:
saptrap_nt_5arm.gb
saptrap_ct_5arm.gb
saptrap_3arm.gb
Protocol for constructing repair templates with our Gibson/ccdB-based method.
Protocol for injection and isolation of strains with SEC selection.
Our old unc-119 based selection protocol is available here.
Protocol for constructing repair templates with SapTrap assembly.
Homology arm template files that go with this protocol:
saptrap_nt_5arm.gb
saptrap_ct_5arm.gb
saptrap_3arm.gb
Protocol for constructing repair templates with our Gibson/ccdB-based method.
Protocol for injection and isolation of strains with SEC selection.
Our old unc-119 based selection protocol is available here.