Cas9-triggered homologous recombination
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Only a few steps are required to modify the genome with Cas9-triggered homologous recombination.  All of the protocols you need are right here.
Protocol for constructing repair templates with SapTrap assembly.
          Homology arm template files that go with this protocol: 
          saptrap_nt_5arm.gb
          saptrap_ct_5arm.gb
          saptrap_3arm.gb
Protocol for constructing repair templates with our Gibson/ccdB-based method.
Protocol for injection and isolation of strains with SEC selection. 

Our old unc-119 based selection protocol is available here.
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