LOCUS       his_72_GFP_repai       10806 bp ds-DNA     circular     13-DEC-2013
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     Note a unique feature of this particular construct:  
COMMENT     Because his-72 and the downstream Y57B8A.33 gene are oriented on
            the same strand, the his-72 3'UTR sequence might be part of the
            Y57B8A.33 promoter.  To avoid disrupting this promoter, we
            duplicated the his-72 3'UTR, with one copy immediately after the
            GFP and a second copy downstream of unc-119(+).  One of the copies
            is located between the LoxP sites so that it will be removed along
            with the unc-119(+) cassette. 
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_recomb     join(792..1761,1784..1878,2764..3215,3219..3229,3231..3233,
                     3268..3310,3313..3530,5956..7624)
                     /label=Genomic Sequence
                     /ApEinfo_fwdcolor=#cccccc
                     /ApEinfo_revcolor=#cccccc
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     join(5693..5735,5738..5905)
                     /label=Genomic Sequence(1)
                     /ApEinfo_label=Genomic Sequence
                     /ApEinfo_fwdcolor=#cccccc
                     /ApEinfo_revcolor=#cccccc
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     5906..5955
                     /label=Genomic Sequence(2)
                     /ApEinfo_label=Genomic Sequence
                     /ApEinfo_fwdcolor=#cccccc
                     /ApEinfo_revcolor=#cccccc
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             join(2764..3045,3105..3233)
                     /label=HIS-72 (a isoform)
                     /ApEinfo_fwdcolor=#00cccc
                     /ApEinfo_revcolor=#006666
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             join(4519..4575,4671..4786,4830..5206,5258..5364)
                     /label=Cbri unc-119
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     5659..5692
                     /label=LoxP
                     /ApEinfo_fwdcolor=steel blue
                     /ApEinfo_revcolor=orange
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             join(6007..6058,6436..6581,7083..7203)
                     /label=Y57B8A.33
                     /ApEinfo_fwdcolor=#00cccc
                     /ApEinfo_revcolor=#006666
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     3'UTR           join(3268..3310,3313..3480)
                     /label=his-72 3'UTR
                     /ApEinfo_fwdcolor=#cc3333
                     /ApEinfo_revcolor=#cc3333
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     3'UTR           join(5693..5735,5738..5905)
                     /label=his-72 3'UTR(1)
                     /ApEinfo_label=his-72 3'UTR
                     /ApEinfo_fwdcolor=#cc3333
                     /ApEinfo_revcolor=#cc3333
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        3531..4518
                     /label=unc-119 Promoter
                     /ApEinfo_fwdcolor=#33cc00
                     /ApEinfo_revcolor=#33cc00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     3'UTR           5365..5658
                     /label=unc-119 terminator
                     /ApEinfo_fwdcolor=#cc3333
                     /ApEinfo_revcolor=#cc3333
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(3646..3665)
                     /label=unc119 5' out
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=#00cc99
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             3752..4222
                     /translation="MLKLTQIKLHDKFIALFNSSWLIICYSVPILSAPYTPLHTLSSF
                     LSWIVSSSPQLCYFYDLRPLLFRSFLSSLTSSSASFLLPFSLLNLPLFPISVNHSKKK
                     KKKTLIHFIFFSVGVCCSVKSELSIPRLLLFLLRFESITPPKRIRFWDSPRGSQ*"
                     /label=ORF frame 1
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     337..791
                     /label=Genomic Sequence(3)
                     /ApEinfo_label=Genomic Sequence
                     /ApEinfo_fwdcolor=#cccccc
                     /ApEinfo_revcolor=#cccccc
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     1764..1783
                     /label=Genomic Sequence(4)
                     /ApEinfo_label=Genomic Sequence
                     /ApEinfo_fwdcolor=#cccccc
                     /ApEinfo_revcolor=#cccccc
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     5530..5549
                     /label=unc119 3' out
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_signal     complement(1765..1783)
                     /label=Cas9 target sequence
                     /ApEinfo_fwdcolor=#ff6600
                     /ApEinfo_revcolor=#ff6600
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     3234..3267
                     /label=LoxP(1)
                     /ApEinfo_label=LoxP
                     /ApEinfo_fwdcolor=steel blue
                     /ApEinfo_revcolor=orange
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_difference 1762..1763
                     /label=PAM Mutations to block Cas9 cleavage of repair
                     template
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_difference 5736..5737
                     /label=PAM Mutations to block Cas9 cleavage of repair temp
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_difference 3311..3312
                     /label=PAM Mutations to block Cas9 cleavage of repair
                     temp(1)
                     /ApEinfo_label=PAM Mutations to block Cas9 cleavage of
                     repair temp
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_signal     complement(3314..3340)
                     /label=Cas9 target sequence(1)
                     /ApEinfo_label=Cas9 target sequence
                     /ApEinfo_fwdcolor=#ff6600
                     /ApEinfo_revcolor=#ff6600
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_signal     complement(5739..5765)
                     /label=Cas9 target sequence(2)
                     /ApEinfo_label=Cas9 target sequence
                     /ApEinfo_fwdcolor=#ff6600
                     /ApEinfo_revcolor=#ff6600
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             join(1879..2049,2101..2254,2306..2469,2521..2745)
                     /label=GFP
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC
       61 ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC
      121 TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA
      181 TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCTAT
      241 TTAGGTGACG CGTTAGAATA CTCAAGCTAT GCATCAAGCT TGGTACCGAG CTCGGATCCA
      301 CTAGTAACGG CCGCCAGTGT GCTGGAATTC GCCCTTagag aagcggcacg catttcggaa
      361 atagtgtgtg tttgcaagag agtggccccc cgattaaact agcgcgcgta aaatttttgc
      421 attttgttgt ttcgttccgt ctggaaaatt cgaatttcgc agcattttaa ttcatatctc
      481 agctcatttc gcatattttt tcctgaaaat ttccagtttg gtccgttacc ttattccggt
      541 taatatactc gaatttcata aaaatctacg gatgaatcgc taagttatcg ctagaaatga
      601 aattctttaa aaaattttgc ccgattagta atttttctga aaatccacgt ggattcagga
      661 tccttcgcga cctgaagttt aaagttaatt ttccagattt tcaatgagga tttttgaaag
      721 atttgatatt tcttggcata agttttccag ctttaatccg gatttggtcc agaccccaca
      781 aaatcgatac gacaaaaggg gggaggaggc ggagacttgg cacaatgcca agtctttgct
      841 ctctgtgtcg tcacgtgtct tcgtgttggc tgagtatcaa acgttatagt gtggacacca
      901 atttggcttc gtacttctta ctcgttcccg ccccgccccg acaccattcg aatatatatt
      961 gtcagttgtt ctgtttgtcg tcgtgatgag aggtgcgtgc gtgtgcgcgc gcgctctccc
     1021 aacttttcgg accattttgc gggctttata ctgcaatttg gctcatttct tctccttttt
     1081 tttccacagg cttattttgt actctcgagt tgacagcctt tttcggtata ggttggaagt
     1141 agtccattaa agtagatgtt tcttaagaat ttatcggctt agcatcgatt tcgacctagt
     1201 tagatgccac gtttatgcgc gaagcggccg cgtcgcggcg agaatttcag tgcggtttta
     1261 cgggattatc cattaaaatc aggaaaatca gcagaaaact tcttaacaaa actaattttt
     1321 ttagaaaaga atttagaaat acgtagtctt gaataattca gcaacttttt caaattttat
     1381 gagaatttcg caatttccta cacctcctat ttcatcatca cttccgaaat cctacgttat
     1441 cgatcgaaca cgcgacgcga ccgcagcgca cctactaagc gcgcgcattt cgaccgtgac
     1501 gcgaccgcaa cgcggtggtg tcagggtgcg cgcgcttttt caatcatatg tggtgcggtc
     1561 gattccttca ttcatccttc tgtatgcagc tgctcatcag tttattgagc atctgctgta
     1621 ccaattcgta ctttaaatta aatattaaga gtacattttc tgtgtgctct gtatataaat
     1681 gtctagaata aactgcaaac aagaattctt ggctccatct ttccctgaat caatatcgat
     1741 cgatagtgac acgcgacgcg aAAgcaacgc gtagggccat ttcatattga ttcttcttct
     1801 actcttcctt cttctccttc tgctctcaca tttcctcact attccaacca ttctcaattc
     1861 tcaatttcca gaacaacaAT GAGTAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT
     1921 CTTGTTGAAT TAGATGGTGA TGTTAATGGG CACAAATTTT CTGTCAGTGG AGAGGGTGAA
     1981 GGTGATGCAA CATACGGAAA ACTTACCCTT AAATTTATTT GCACTACTGG AAAACTACCT
     2041 GTTCCATGGG TAAGTTTAAA CATATATATA CTAACTAACC CTGATTATTT AAATTTTCAG
     2101 CCAACACTTG TCACTACTTT CTGTTATGGT GTTCAATGCT TCTCGAGATA CCCAGATCAT
     2161 ATGAAACGGC ATGACTTTTT CAAGAGTGCC ATGCCCGAAG GTTATGTACA GGAAAGAACT
     2221 ATATTTTTCA AAGATGACGG GAACTACAAG ACACGTAAGT TTAAACAGTT CGGTACTAAC
     2281 TAACCATACA TATTTAAATT TTCAGGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT
     2341 AATAGAATCG AGTTAAAAGG TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA
     2401 TTGGAATACA ACTATAACTC ACACAATGTA TACATCATGG CAGACAAACA AAAGAATGGA
     2461 ATCAAAGTTG TAAGTTTAAA CATGATTTTA CTAACTAACT AATCTGATTT AAATTTTCAG
     2521 AACTTCAAAA TTAGACACAA CATTGAAGAT GGAAGCGTTC AACTAGCAGA CCATTATCAA
     2581 CAAAATACTC CAATTGGCGA TGGCCCTGTC CTTTTACCAG ACAACCATTA CCTGTCCACA
     2641 CAATCTGCCC TTTCGAAAGA TCCCAACGAA AAGAGAGACC ACATGGTCCT TCTTGAGTTT
     2701 GTAACAGCTG CTGGGATTAC ACATGGCATG GATGAACTAT ACAAAtcagg agctagcgga
     2761 gccATGGCTC GTACCAAGCA AACCGCTCGT AAATCCACCG GAGGAAAGGC TCCAAGAAAG
     2821 CAGTTGGCCA CCAAGGCCGC CCGCAAATCG GCTCCAACCA CCGGAGGAGT CAAGAAGCCA
     2881 CATCGTTACC GTCCAGGAAC TGTCGCTCTC CGTGAGATTC GTCGTTACCA GAAGTCGACT
     2941 GAGCTTCTCA TCCGCAAGCT TCCTTTCCAA CGTCTCGTTC GTGAGATTGC CCAGGACTTC
     3001 AAGACTGATC TCCGCTTCCA GTCGGCTGCC ATCGGAGCTC TCCAGgtttg tagtttgaat
     3061 ttgaaattta aaaaaaatta attaaaatct tttttttttt ccagGAGGCA TCTGAAGCAT
     3121 ACCTCGTCGG ACTCTTCGAG GACACCAACC TGTGCGCCAT CCACGCCAAG CGCGTCACCA
     3181 TCATGCCAAA GGACATGCAA CTCGCCAGAC GCATCagaGG AGAACGTGCc TAAATAACTT
     3241 CGTATAGCAT ACATTATACG AAGTTATgct ccatcaccaa ttctcgaagc acttttaata
     3301 ttcatttaat AAaacttgta ccataatcct cgtatttttc catcccgaaa attgattttt
     3361 gcgatcgaac cgccgatctc atcaccactt ttctctccgt tctttatgtc ttattttatt
     3421 cgtaaattgc tgaatttcat gttgaactcc aacaaaaaaa aataaagttt cgtgtgcttc
     3481 agtagtctag caacatcata tgaacgccta gcaactatat tccacgccgc CTTTGAGCCA
     3541 ATTTATCCAA GTCCTTGTAA AAAGTATTCG AAAATTGTTA ACGGATAAAA TGTTTATTAT
     3601 AATATCAAAA ACAATTTGTC AGTTGACCAC TTTTTGATAT ATTTGACAGA AACCGGGATG
     3661 AATTGGCTCA AAGTTAGGGC GCCCTTCTAT TACAGGGTTT CTGATAACAA ACCGGTTATT
     3721 AACTCCCAAC AAGGGATGAT TTCAATTCAT CATGCTCAAA TTGACCCAAA TTAAGTTACA
     3781 TGACAAATTC ATCGCCCTTT TCAACTCTTC TTGGCTCATC ATCTGTTATT CTGTTCCTAT
     3841 TCTGTCTGCA CCCTATACCC CTTTGCATAC TCTCTCGTCA TTCCTTTCTT GGATAGTGTC
     3901 TTCTTCTCCC CAGCTCTGCT ACTTCTATGA CTTGCGCCCG CTGCTTTTCC GCTCGTTCCT
     3961 CTCCTCTCTG ACGTCTTCTT CTGCTTCTTT TCTCCTTCCT TTTTCCCTTC TCAATCTTCC
     4021 TCTTTTTCCC ATTTCTGTCA ATCATTCGAA GAAGAAGAAG AAGAAGACCC TCATTCATTT
     4081 CATTTTTTTT TCTGTCGGTG TGTGCTGCTC GGTTAAGAGT GAGCTCTCTA TTCCACGTCT
     4141 TCTTCTTTTT CTTCTTCGAT TCGAATCAAT CACTCCACCA AAACGCATTC GTTTTTGGGA
     4201 TTCACCCCGC GGTTCGCAAT AGGTTTCTTT TTCAAATTAT TAGCGTTATA AATAGAAAAA
     4261 TGGTGGAGTT TCAATAAAAA TGATAATTTA CAAAGTGTAT TTTGATTACA TGTACTCAAA
     4321 AAGGTTGAAA TTTTTCATAC CAGTTTTCCG GAAATCCATC TGATATCATT ATCGTATTTT
     4381 TCTTTTTAAA AATGTTTTCA AAAAAAAACA AAATATAGCT GGTTATTTGG CACCCTCTAA
     4441 TTACCATTTT CCTGTCACAC CACACTCTTT TCCTTCTTCC ACTTCTTTCC GTTTTCAGCC
     4501 GCTTCCAACC AAACCGATAT GAAAGCCGAG CAACAACGAT CGATTCCACC CGGCTCGGCG
     4561 ACCTTCCCGT CGCAGGTGAG ACTCAGAAAA CTAGAGAAAC CGCTCAACTA ACTCTTGATA
     4621 TCCGATTTCA TTCTTTTCTC TTTTCTTTTT GTTGAACTTT TCCATATTTC CAGATGCCAC
     4681 GGCCACCACC AAGCACCGAA CAAGGAATCA CAACGGAATC GGAGCTTGCG AAGAAAGCTC
     4741 AAATCACTCC GAACGACGTT TTAGCACTTC CGGGAATCAC TCAAGGTATC TTGCTCCCTT
     4801 TGGATTTCAG TTTAAAACAT AAATTTAGGA TTCTTATGCT CCCCATCTGC GAACATCTAT
     4861 AACATCGAGT TCACCAAGTT CCAAATCCGT GATCTGGACA CTGAGCAAGT GCTTTTCGAG
     4921 ATCGCCAAAC CGGAGAACGA TCAGGAGAAT GATGAGTCGC CACAGGAGTC GGCAAGATAC
     4981 GTGCGTTATA GATTTGCTCC AAACTTTTTG AAACTCAAAA CGGTCGGAGC AACTGTGGAA
     5041 TTCAAAGTAG GAGACATCCC AATCCATCAT TTCCGAATGA TCGAACGTCA CTTCTTCAAA
     5101 GATCGCCTTC TGAAGTGTTT TGACTTTGAA TTCGGATTCT GTATTCCGAA TTCACGAAAC
     5161 AACTGTGAAC ATATCTATGA GTTCCCTCAA CTCTCTCAAC AACTCAGTGA GTCATTATTC
     5221 TAAAAAGTAC AATTCAAAAG ACTAATCTCT TTTTCAGTGG ACGACATGAT CAACAATCCA
     5281 AACGAGACTC GTTCTGACAG CTTCTATTTC GTCGATAACA AACTCGTCAT GCACAACAAA
     5341 GCCGACTACT CATATGATGC ATAAATATTT AATACAAAAA TGTTCTGGAT AATTATTCTG
     5401 TCGAATAGAA AAAAAACTCC AAATGTGATT AAATTCCAAT AATTCCTGTC TAGTTTGTTC
     5461 CTTCCCCTTC CCCTTCTCAT GTTCAATGCA TTCCTAAGCT TTTCAGTTCC CCCCTTGTTT
     5521 CTATATTTTT TCGCTGTCCT GTCACACTCG CTAAAAACAC TAATCACACG GAAATCTGTT
     5581 TTCAATAAAA ACTCCAACTT TAACTCATTT TCAATTTCAA CTGAAAGATT TTTTCATTAG
     5641 AGAATGTCTA GAACTAGGAT AACTTCGTAT AGCATACATT ATACGAAGTT ATgctccatc
     5701 accaattctc gaagcacttt taatattcat ttaatAAaac ttgtaccata atcctcgtat
     5761 ttttccatcc cgaaaattga tttttgcgat cgaaccgccg atctcatcac cacttttctc
     5821 tccgttcttt atgtcttatt ttattcgtaa attgctgaat ttcatgttga actccaacaa
     5881 aaaaaaataa agtttcgtgt gcttcagtag tctagcaaca tcatatgaac gcctagcaac
     5941 tatattccac gccgccgcag ttttgaattg aattcactaa attcatgctc tgaattcaca
     6001 aaaaaaATGT TTCTTCGAGT CATTGGAAAT AGTAAAAAAC GCCAGCGACT ATTTATCAgt
     6061 aagttttaaa agggtctcgt cgcgaaattc acattaaaaa tattaatttt ctgtatgtac
     6121 ctttaaaatt actgtagaaa atctatcaga tgggtcttgg cgcgcaaaac gcaaaaaata
     6181 aattgatttc attgcatgct ttttccgcgc cgagacccat ctaacctaaa tttttcaaaa
     6241 aggtgtgcgc ctttaaagat tactgtagtt tcgaacttgt gtagttgcgg agttttcatc
     6301 gatttttttg taaataaata gttttatttt agtttttgaa gaaataaata ttttttcaac
     6361 gaacaactat atgaaaaatc gataaaaatc ccgcaatttt ttggaaaatt ctgtgatgga
     6421 taataccaaa ttcagATGTT TCCTTCTTTT TTCTCATCAT TGGGCTTCTT GTTCTCGGTG
     6481 TCTTCACAAT CAAAAAGAAC ACCTATATCA CCTTCGATTC ATGGGATCTA GCACAAGAGA
     6541 TTGCTCAGAA CGCGACGGAT GCAGCGATGG AAAACTCTTC Tgtaagtttt tcacggaatt
     6601 ctggcctccc tcataaatcg aaatggcaga gtttgccgaa ctaggccatt ttgagtccgc
     6661 gagattttgt gtaggtttac ggcgcgttgc gtgtcgcgtc gcggctcgtt tttagttgta
     6721 aaaccgatgt atttgtccgt gtggagtaca ctacactttt ccacgcgttg tccggcaggc
     6781 gattgccaat ggagcgcgaa aattcaatga gaaatgccag aaacccgtgt ttttttgcac
     6841 ggatttctgg cttccctcat aaattgaaat ggaagagttt tttgccgaac taggccaggc
     6901 catatctggg gtagatttac ggcgcgttgc gtgtcgcgtc gcggttcgat tgtaaaacga
     6961 aatgcatttg tccgtgtgga gtacacgact ttcccagggc gattgtcaat ggagcgcgaa
     7021 caattcaatg aggaaggcca gaagcccgtg aatttgaaaa aaaaactaaa tttcaattat
     7081 agCACATTGA TTTCTCTACT GTTCTGTGGA ATGCTGACAT GGAATTGGTT TTAGCGAATA
     7141 AAACGATTGA TGGGCCTCCG AAAAGAGATA TTCTATTCGT TGTCAGTACT TTTATCGGAG
     7201 TTGgtaagtt gaattcgata tgccatatta ttagttttat aaaatactta ttacggagac
     7261 acaaaattct gaaaatgcgt attgcgcaac gcattttacg ctcaaaatat ctcgtagcga
     7321 aaagaaaact acagtaattc tttaaatgac tactgtagcg cttgtgtcga tttacgggct
     7381 ccatcagcga tattctattg tctttgggtt cttcgtatta atttttttcc atttctttac
     7441 ctaatttgaa tgttctaaaa ataaataaac cgtaaatcca cactacaata gtcattgaaa
     7501 gaattactgt agttttcttt tcgctacgag atattttgcg cgtcaaatat gttgcacaat
     7561 acgcattctt aaaatttttc cggtaatatg agcccattta cctgcctcag agcaaattca
     7621 gcctAAGGGC GAATTCTGCA GATATCCATC ACACTGGCGG CCGCTCGAGC ATGCATCTAG
     7681 AGGGCCCAAT TCGCCCTATA GTGAGTCGTA TTACAATTCA CTGGCCGTCG TTTTACAACG
     7741 TCGTGACTGG GAAAACCCTG GCGTTACCCA ACTTAATCGC CTTGCAGCAC ATCCCCCTTT
     7801 CGCCAGCTGG CGTAATAGCG AAGAGGCCCG CACCGATCGC CCTTCCCAAC AGTTGCGCAG
     7861 CCTATACGTA CGGCAGTTTA AGGTTTACAC CTATAAAAGA GAGAGCCGTT ATCGTCTGTT
     7921 TGTGGATGTA CAGAGTGATA TTATTGACAC GCCGGGGCGA CGGATGGTGA TCCCCCTGGC
     7981 CAGTGCACGT CTGCTGTCAG ATAAAGTCTC CCGTGAACTT TACCCGGTGG TGCATATCGG
     8041 GGATGAAAGC TGGCGCATGA TGACCACCGA TATGGCCAGT GTGCCGGTCT CCGTTATCGG
     8101 GGAAGAAGTG GCTGATCTCA GCCACCGCGA AAATGACATC AAAAACGCCA TTAACCTGAT
     8161 GTTCTGGGGA ATATAAATGT CAGGCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT
     8221 TTTCACGTAG AAAGCCAGTC CGCAGAAACG GTGCTGACCC CGGATGAATG TCAGCTACTG
     8281 GGCTATCTGG ACAAGGGAAA ACGCAAGCGC AAAGAGAAAG CAGGTAGCTT GCAGTGGGCT
     8341 TACATGGCGA TAGCTAGACT GGGCGGTTTT ATGGACAGCA AGCGAACCGG AATTGCCAGC
     8401 TGGGGCGCCC TCTGGTAAGG TTGGGAAGCC CTGCAAAGTA AACTGGATGG CTTTCTTGCC
     8461 GCCAAGGATC TGATGGCGCA GGGGATCAAG CTCTGATCAA GAGACAGGAT GAGGATCGTT
     8521 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT
     8581 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT
     8641 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA
     8701 ACTGCAAGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC
     8761 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG
     8821 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC
     8881 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA
     8941 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA
     9001 CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGAGCATGCC
     9061 CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA
     9121 AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA
     9181 GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG
     9241 CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT
     9301 TCTTGACGAG TTCTTCTGAA TTATTAACGC TTACAATTTC CTGATGCGGT ATTTTCTCCT
     9361 TACGCATCTG TGCGGTATTT CACACCGCAT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG
     9421 GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT
     9481 AACCCTGATA AATGCTTCAA TAATAGCACG TGAGGAGGGC CACCATGGCC AAGTTGACCA
     9541 GTGCCGTTCC GGTGCTCACC GCGCGCGACG TCGCCGGAGC GGTCGAGTTC TGGACCGACC
     9601 GGCTCGGGTT CTCCCGGGAC TTCGTGGAGG ACGACTTCGC CGGTGTGGTC CGGGACGACG
     9661 TGACCCTGTT CATCAGCGCG GTCCAGGACC AGGTGGTGCC GGACAACACC CTGGCCTGGG
     9721 TGTGGGTGCG CGGCCTGGAC GAGCTGTACG CCGAGTGGTC GGAGGTCGTG TCCACGAACT
     9781 TCCGGGACGC CTCCGGGCCG GCCATGACCG AGATCGGCGA GCAGCCGTGG GGGCGGGAGT
     9841 TCGCCCTGCG CGACCCGGCC GGCAACTGCG TGCACTTCGT GGCCGAGGAG CAGGACTGAC
     9901 ACGTGCTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC
     9961 TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA
    10021 AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA
    10081 AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC
    10141 CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT
    10201 AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC
    10261 TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC
    10321 GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA
    10381 GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG
    10441 CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG
    10501 GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT
    10561 TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT
    10621 GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC
    10681 ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT
    10741 GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG
    10801 CGGAAG     
//
