LOCUS       his_72_GFP_repai       10371 bp ds-DNA     circular     12-DEC-2013
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     Note a unique feature of this particular construct:  
COMMENT     Because his-72 and the downstream Y57B8A.33 gene are oriented on
            the same strand, the his-72 3'UTR sequence might be part of the
            Y57B8A.33 promoter.  To avoid disrupting this promoter, we
            duplicated the his-72 3'UTR, with one copy immediately after the
            GFP and a second copy downstream of unc-119(+).  One of the copies
            is located between the LoxP sites so that it will be removed along
            with the unc-119(+) cassette. 
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_recomb     join(337..1895,1899..1909,2796..2798,2833..2875,2878..3095,
                     5521..7189)
                     /label=Genomic Sequence
                     /ApEinfo_fwdcolor=#cccccc
                     /ApEinfo_revcolor=#cccccc
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     join(5258..5300,5303..5470)
                     /label=Genomic Sequence(1)
                     /ApEinfo_label=Genomic Sequence
                     /ApEinfo_fwdcolor=#cccccc
                     /ApEinfo_revcolor=#cccccc
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     5471..5520
                     /label=Genomic Sequence(2)
                     /ApEinfo_label=Genomic Sequence
                     /ApEinfo_fwdcolor=#cccccc
                     /ApEinfo_revcolor=#cccccc
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             join(1444..1725,1785..1910,2796..2798)
                     /label=HIS-72 (a isoform)
                     /ApEinfo_fwdcolor=#00cccc
                     /ApEinfo_revcolor=#006666
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             join(4084..4140,4236..4351,4395..4771,4823..4929)
                     /label=Cbri unc-119
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     5224..5257
                     /label=LoxP
                     /ApEinfo_fwdcolor=steel blue
                     /ApEinfo_revcolor=orange
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             join(5572..5623,6001..6146,6648..6768)
                     /label=Y57B8A.33
                     /ApEinfo_fwdcolor=#00cccc
                     /ApEinfo_revcolor=#006666
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     337..356
                     /label=his-72 Homology L #215
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=#00cc99
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(7171..7189)
                     /label=his-72 Homology R #216
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=#00cc99
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     3'UTR           join(2833..2875,2878..3045)
                     /label=his-72 3'UTR
                     /ApEinfo_fwdcolor=#cc3333
                     /ApEinfo_revcolor=#cc3333
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     3'UTR           join(5258..5300,5303..5470)
                     /label=his-72 3'UTR(1)
                     /ApEinfo_label=his-72 3'UTR
                     /ApEinfo_fwdcolor=#cc3333
                     /ApEinfo_revcolor=#cc3333
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             join(1929..2099,2151..2304,2356..2519,2571..2795)
                     /label=GFP
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        3096..4083
                     /label=unc-119 Promoter
                     /ApEinfo_fwdcolor=#33cc00
                     /ApEinfo_revcolor=#33cc00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     3'UTR           4930..5223
                     /label=unc-119 terminator
                     /ApEinfo_fwdcolor=#cc3333
                     /ApEinfo_revcolor=#cc3333
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(3211..3230)
                     /label=unc119 5' out
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=#00cc99
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             3317..3787
                     /translation="MLKLTQIKLHDKFIALFNSSWLIICYSVPILSAPYTPLHTLSSF
                     LSWIVSSSPQLCYFYDLRPLLFRSFLSSLTSSSASFLLPFSLLNLPLFPISVNHSKKK
                     KKKTLIHFIFFSVGVCCSVKSELSIPRLLLFLLRFESITPPKRIRFWDSPRGSQ*"
                     /label=ORF frame 1
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     5095..5114
                     /label=unc119 3' out
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     2799..2832
                     /label=LoxP(1)
                     /ApEinfo_label=LoxP
                     /ApEinfo_fwdcolor=steel blue
                     /ApEinfo_revcolor=orange
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_difference 5301..5302
                     /label=PAM Mutations to block Cas9 cleavage of repair
                     template
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_difference 2876..2877
                     /label=PAM Mutations to block Cas9 cleavage of repair
                     template(1)
                     /ApEinfo_label=PAM Mutations to block Cas9 cleavage of
                     repair template
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_signal     complement(2879..2905)
                     /label=Cas9 target sequence
                     /ApEinfo_fwdcolor=#ff6600
                     /ApEinfo_revcolor=#ff6600
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_signal     complement(5304..5330)
                     /label=Cas9 target sequence(1)
                     /ApEinfo_label=Cas9 target sequence
                     /ApEinfo_fwdcolor=#ff6600
                     /ApEinfo_revcolor=#ff6600
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC
       61 ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC
      121 TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA
      181 TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCTAT
      241 TTAGGTGACG CGTTAGAATA CTCAAGCTAT GCATCAAGCT TGGTACCGAG CTCGGATCCA
      301 CTAGTAACGG CCGCCAGTGT GCTGGAATTC GCCCTTgacc ccacaaaatc gatacgacaa
      361 aaggggggag gaggcggaga cttggcacaa tgccaagtct ttgctctctg tgtcgtcacg
      421 tgtcttcgtg ttggctgagt atcaaacgtt atagtgtgga caccaatttg gcttcgtact
      481 tcttactcgt tcccgccccg ccccgacacc attcgaatat atattgtcag ttgttctgtt
      541 tgtcgtcgtg atgagaggtg cgtgcgtgtg cgcgcgcgct ctcccaactt ttcggaccat
      601 tttgcgggct ttatactgca atttggctca tttcttctcc ttttttttcc acaggcttat
      661 tttgtactct cgagttgaca gcctttttcg gtataggttg gaagtagtcc attaaagtag
      721 atgtttctta agaatttatc ggcttagcat cgatttcgac ctagttagat gccacgttta
      781 tgcgcgaagc ggccgcgtcg cggcgagaat ttcagtgcgg ttttacggga ttatccatta
      841 aaatcaggaa aatcagcaga aaacttctta acaaaactaa tttttttaga aaagaattta
      901 gaaatacgta gtcttgaata attcagcaac tttttcaaat tttatgagaa tttcgcaatt
      961 tcctacacct cctatttcat catcacttcc gaaatcctac gttatcgatc gaacacgcga
     1021 cgcgaccgca gcgcacctac taagcgcgcg catttcgacc gtgacgcgac cgcaacgcgg
     1081 tggtgtcagg gtgcgcgcgc tttttcaatc atatgtggtg cggtcgattc cttcattcat
     1141 ccttctgtat gcagctgctc atcagtttat tgagcatctg ctgtaccaat tcgtacttta
     1201 aattaaatat taagagtaca ttttctgtgt gctctgtata taaatgtcta gaataaactg
     1261 caaacaagaa ttcttggctc catctttccc tgaatcaata tcgatcgata gtgacacgcg
     1321 acgcgaccgc aacgcgtagg gccatttcat attgattctt cttctactct tccttcttct
     1381 ccttctgctc tcacatttcc tcactattcc aaccattctc aattctcaat ttccagaaca
     1441 acaATGGCTC GTACCAAGCA AACCGCTCGT AAATCCACCG GAGGAAAGGC TCCAAGAAAG
     1501 CAGTTGGCCA CCAAGGCCGC CCGCAAATCG GCTCCAACCA CCGGAGGAGT CAAGAAGCCA
     1561 CATCGTTACC GTCCAGGAAC TGTCGCTCTC CGTGAGATTC GTCGTTACCA GAAGTCGACT
     1621 GAGCTTCTCA TCCGCAAGCT TCCTTTCCAA CGTCTCGTTC GTGAGATTGC CCAGGACTTC
     1681 AAGACTGATC TCCGCTTCCA GTCGGCTGCC ATCGGAGCTC TCCAGgtttg tagtttgaat
     1741 ttgaaattta aaaaaaatta attaaaatct tttttttttt ccagGAGGCA TCTGAAGCAT
     1801 ACCTCGTCGG ACTCTTCGAG GACACCAACC TGTGCGCCAT CCACGCCAAG CGCGTCACCA
     1861 TCATGCCAAA GGACATGCAA CTCGCCAGAC GCATCagaGG AGAACGTGCc tcaggagcta
     1921 gcggagccAT GAGTAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT CTTGTTGAAT
     1981 TAGATGGTGA TGTTAATGGG CACAAATTTT CTGTCAGTGG AGAGGGTGAA GGTGATGCAA
     2041 CATACGGAAA ACTTACCCTT AAATTTATTT GCACTACTGG AAAACTACCT GTTCCATGGG
     2101 TAAGTTTAAA CATATATATA CTAACTAACC CTGATTATTT AAATTTTCAG CCAACACTTG
     2161 TCACTACTTT CTGTTATGGT GTTCAATGCT TCTCGAGATA CCCAGATCAT ATGAAACGGC
     2221 ATGACTTTTT CAAGAGTGCC ATGCCCGAAG GTTATGTACA GGAAAGAACT ATATTTTTCA
     2281 AAGATGACGG GAACTACAAG ACACGTAAGT TTAAACAGTT CGGTACTAAC TAACCATACA
     2341 TATTTAAATT TTCAGGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT AATAGAATCG
     2401 AGTTAAAAGG TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA TTGGAATACA
     2461 ACTATAACTC ACACAATGTA TACATCATGG CAGACAAACA AAAGAATGGA ATCAAAGTTG
     2521 TAAGTTTAAA CATGATTTTA CTAACTAACT AATCTGATTT AAATTTTCAG AACTTCAAAA
     2581 TTAGACACAA CATTGAAGAT GGAAGCGTTC AACTAGCAGA CCATTATCAA CAAAATACTC
     2641 CAATTGGCGA TGGCCCTGTC CTTTTACCAG ACAACCATTA CCTGTCCACA CAATCTGCCC
     2701 TTTCGAAAGA TCCCAACGAA AAGAGAGACC ACATGGTCCT TCTTGAGTTT GTAACAGCTG
     2761 CTGGGATTAC ACATGGCATG GATGAACTAT ACAAATAAAT AACTTCGTAT AGCATACATT
     2821 ATACGAAGTT ATgctccatc accaattctc gaagcacttt taatattcat ttaatAAaac
     2881 ttgtaccata atcctcgtat ttttccatcc cgaaaattga tttttgcgat cgaaccgccg
     2941 atctcatcac cacttttctc tccgttcttt atgtcttatt ttattcgtaa attgctgaat
     3001 ttcatgttga actccaacaa aaaaaaataa agtttcgtgt gcttcagtag tctagcaaca
     3061 tcatatgaac gcctagcaac tatattccac gccgcCTTTG AGCCAATTTA TCCAAGTCCT
     3121 TGTAAAAAGT ATTCGAAAAT TGTTAACGGA TAAAATGTTT ATTATAATAT CAAAAACAAT
     3181 TTGTCAGTTG ACCACTTTTT GATATATTTG ACAGAAACCG GGATGAATTG GCTCAAAGTT
     3241 AGGGCGCCCT TCTATTACAG GGTTTCTGAT AACAAACCGG TTATTAACTC CCAACAAGGG
     3301 ATGATTTCAA TTCATCATGC TCAAATTGAC CCAAATTAAG TTACATGACA AATTCATCGC
     3361 CCTTTTCAAC TCTTCTTGGC TCATCATCTG TTATTCTGTT CCTATTCTGT CTGCACCCTA
     3421 TACCCCTTTG CATACTCTCT CGTCATTCCT TTCTTGGATA GTGTCTTCTT CTCCCCAGCT
     3481 CTGCTACTTC TATGACTTGC GCCCGCTGCT TTTCCGCTCG TTCCTCTCCT CTCTGACGTC
     3541 TTCTTCTGCT TCTTTTCTCC TTCCTTTTTC CCTTCTCAAT CTTCCTCTTT TTCCCATTTC
     3601 TGTCAATCAT TCGAAGAAGA AGAAGAAGAA GACCCTCATT CATTTCATTT TTTTTTCTGT
     3661 CGGTGTGTGC TGCTCGGTTA AGAGTGAGCT CTCTATTCCA CGTCTTCTTC TTTTTCTTCT
     3721 TCGATTCGAA TCAATCACTC CACCAAAACG CATTCGTTTT TGGGATTCAC CCCGCGGTTC
     3781 GCAATAGGTT TCTTTTTCAA ATTATTAGCG TTATAAATAG AAAAATGGTG GAGTTTCAAT
     3841 AAAAATGATA ATTTACAAAG TGTATTTTGA TTACATGTAC TCAAAAAGGT TGAAATTTTT
     3901 CATACCAGTT TTCCGGAAAT CCATCTGATA TCATTATCGT ATTTTTCTTT TTAAAAATGT
     3961 TTTCAAAAAA AAACAAAATA TAGCTGGTTA TTTGGCACCC TCTAATTACC ATTTTCCTGT
     4021 CACACCACAC TCTTTTCCTT CTTCCACTTC TTTCCGTTTT CAGCCGCTTC CAACCAAACC
     4081 GATATGAAAG CCGAGCAACA ACGATCGATT CCACCCGGCT CGGCGACCTT CCCGTCGCAG
     4141 GTGAGACTCA GAAAACTAGA GAAACCGCTC AACTAACTCT TGATATCCGA TTTCATTCTT
     4201 TTCTCTTTTC TTTTTGTTGA ACTTTTCCAT ATTTCCAGAT GCCACGGCCA CCACCAAGCA
     4261 CCGAACAAGG AATCACAACG GAATCGGAGC TTGCGAAGAA AGCTCAAATC ACTCCGAACG
     4321 ACGTTTTAGC ACTTCCGGGA ATCACTCAAG GTATCTTGCT CCCTTTGGAT TTCAGTTTAA
     4381 AACATAAATT TAGGATTCTT ATGCTCCCCA TCTGCGAACA TCTATAACAT CGAGTTCACC
     4441 AAGTTCCAAA TCCGTGATCT GGACACTGAG CAAGTGCTTT TCGAGATCGC CAAACCGGAG
     4501 AACGATCAGG AGAATGATGA GTCGCCACAG GAGTCGGCAA GATACGTGCG TTATAGATTT
     4561 GCTCCAAACT TTTTGAAACT CAAAACGGTC GGAGCAACTG TGGAATTCAA AGTAGGAGAC
     4621 ATCCCAATCC ATCATTTCCG AATGATCGAA CGTCACTTCT TCAAAGATCG CCTTCTGAAG
     4681 TGTTTTGACT TTGAATTCGG ATTCTGTATT CCGAATTCAC GAAACAACTG TGAACATATC
     4741 TATGAGTTCC CTCAACTCTC TCAACAACTC AGTGAGTCAT TATTCTAAAA AGTACAATTC
     4801 AAAAGACTAA TCTCTTTTTC AGTGGACGAC ATGATCAACA ATCCAAACGA GACTCGTTCT
     4861 GACAGCTTCT ATTTCGTCGA TAACAAACTC GTCATGCACA ACAAAGCCGA CTACTCATAT
     4921 GATGCATAAA TATTTAATAC AAAAATGTTC TGGATAATTA TTCTGTCGAA TAGAAAAAAA
     4981 ACTCCAAATG TGATTAAATT CCAATAATTC CTGTCTAGTT TGTTCCTTCC CCTTCCCCTT
     5041 CTCATGTTCA ATGCATTCCT AAGCTTTTCA GTTCCCCCCT TGTTTCTATA TTTTTTCGCT
     5101 GTCCTGTCAC ACTCGCTAAA AACACTAATC ACACGGAAAT CTGTTTTCAA TAAAAACTCC
     5161 AACTTTAACT CATTTTCAAT TTCAACTGAA AGATTTTTTC ATTAGAGAAT GTCTAGAACT
     5221 AGGATAACTT CGTATAGCAT ACATTATACG AAGTTATgct ccatcaccaa ttctcgaagc
     5281 acttttaata ttcatttaat AAaacttgta ccataatcct cgtatttttc catcccgaaa
     5341 attgattttt gcgatcgaac cgccgatctc atcaccactt ttctctccgt tctttatgtc
     5401 ttattttatt cgtaaattgc tgaatttcat gttgaactcc aacaaaaaaa aataaagttt
     5461 cgtgtgcttc agtagtctag caacatcata tgaacgccta gcaactatat tccacgccgc
     5521 cgcagttttg aattgaattc actaaattca tgctctgaat tcacaaaaaa aATGTTTCTT
     5581 CGAGTCATTG GAAATAGTAA AAAACGCCAG CGACTATTTA TCAgtaagtt ttaaaagggt
     5641 ctcgtcgcga aattcacatt aaaaatatta attttctgta tgtaccttta aaattactgt
     5701 agaaaatcta tcagatgggt cttggcgcgc aaaacgcaaa aaataaattg atttcattgc
     5761 atgctttttc cgcgccgaga cccatctaac ctaaattttt caaaaaggtg tgcgccttta
     5821 aagattactg tagtttcgaa cttgtgtagt tgcggagttt tcatcgattt ttttgtaaat
     5881 aaatagtttt attttagttt ttgaagaaat aaatattttt tcaacgaaca actatatgaa
     5941 aaatcgataa aaatcccgca attttttgga aaattctgtg atggataata ccaaattcag
     6001 ATGTTTCCTT CTTTTTTCTC ATCATTGGGC TTCTTGTTCT CGGTGTCTTC ACAATCAAAA
     6061 AGAACACCTA TATCACCTTC GATTCATGGG ATCTAGCACA AGAGATTGCT CAGAACGCGA
     6121 CGGATGCAGC GATGGAAAAC TCTTCTgtaa gtttttcacg gaattctggc ctccctcata
     6181 aatcgaaatg gcagagtttg ccgaactagg ccattttgag tccgcgagat tttgtgtagg
     6241 tttacggcgc gttgcgtgtc gcgtcgcggc tcgtttttag ttgtaaaacc gatgtatttg
     6301 tccgtgtgga gtacactaca cttttccacg cgttgtccgg caggcgattg ccaatggagc
     6361 gcgaaaattc aatgagaaat gccagaaacc cgtgtttttt tgcacggatt tctggcttcc
     6421 ctcataaatt gaaatggaag agttttttgc cgaactaggc caggccatat ctggggtaga
     6481 tttacggcgc gttgcgtgtc gcgtcgcggt tcgattgtaa aacgaaatgc atttgtccgt
     6541 gtggagtaca cgactttccc agggcgattg tcaatggagc gcgaacaatt caatgaggaa
     6601 ggccagaagc ccgtgaattt gaaaaaaaaa ctaaatttca attatagCAC ATTGATTTCT
     6661 CTACTGTTCT GTGGAATGCT GACATGGAAT TGGTTTTAGC GAATAAAACG ATTGATGGGC
     6721 CTCCGAAAAG AGATATTCTA TTCGTTGTCA GTACTTTTAT CGGAGTTGgt aagttgaatt
     6781 cgatatgcca tattattagt tttataaaat acttattacg gagacacaaa attctgaaaa
     6841 tgcgtattgc gcaacgcatt ttacgctcaa aatatctcgt agcgaaaaga aaactacagt
     6901 aattctttaa atgactactg tagcgcttgt gtcgatttac gggctccatc agcgatattc
     6961 tattgtcttt gggttcttcg tattaatttt tttccatttc tttacctaat ttgaatgttc
     7021 taaaaataaa taaaccgtaa atccacacta caatagtcat tgaaagaatt actgtagttt
     7081 tcttttcgct acgagatatt ttgcgcgtca aatatgttgc acaatacgca ttcttaaaat
     7141 ttttccggta atatgagccc atttacctgc ctcagagcaa attcagcctA AGGGCGAATT
     7201 CTGCAGATAT CCATCACACT GGCGGCCGCT CGAGCATGCA TCTAGAGGGC CCAATTCGCC
     7261 CTATAGTGAG TCGTATTACA ATTCACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA
     7321 CCCTGGCGTT ACCCAACTTA ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA
     7381 TAGCGAAGAG GCCCGCACCG ATCGCCCTTC CCAACAGTTG CGCAGCCTAT ACGTACGGCA
     7441 GTTTAAGGTT TACACCTATA AAAGAGAGAG CCGTTATCGT CTGTTTGTGG ATGTACAGAG
     7501 TGATATTATT GACACGCCGG GGCGACGGAT GGTGATCCCC CTGGCCAGTG CACGTCTGCT
     7561 GTCAGATAAA GTCTCCCGTG AACTTTACCC GGTGGTGCAT ATCGGGGATG AAAGCTGGCG
     7621 CATGATGACC ACCGATATGG CCAGTGTGCC GGTCTCCGTT ATCGGGGAAG AAGTGGCTGA
     7681 TCTCAGCCAC CGCGAAAATG ACATCAAAAA CGCCATTAAC CTGATGTTCT GGGGAATATA
     7741 AATGTCAGGC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTCA CGTAGAAAGC
     7801 CAGTCCGCAG AAACGGTGCT GACCCCGGAT GAATGTCAGC TACTGGGCTA TCTGGACAAG
     7861 GGAAAACGCA AGCGCAAAGA GAAAGCAGGT AGCTTGCAGT GGGCTTACAT GGCGATAGCT
     7921 AGACTGGGCG GTTTTATGGA CAGCAAGCGA ACCGGAATTG CCAGCTGGGG CGCCCTCTGG
     7981 TAAGGTTGGG AAGCCCTGCA AAGTAAACTG GATGGCTTTC TTGCCGCCAA GGATCTGATG
     8041 GCGCAGGGGA TCAAGCTCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA
     8101 AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG
     8161 GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG
     8221 CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AAGACGAGGC
     8281 AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT
     8341 CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC
     8401 ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA
     8461 TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC
     8521 ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG
     8581 GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGAGC ATGCCCGACG GCGAGGATCT
     8641 CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC
     8701 TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC
     8761 TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA
     8821 CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT
     8881 CTGAATTATT AACGCTTACA ATTTCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG
     8941 TATTTCACAC CGCATCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT
     9001 TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC
     9061 TTCAATAATA GCACGTGAGG AGGGCCACCA TGGCCAAGTT GACCAGTGCC GTTCCGGTGC
     9121 TCACCGCGCG CGACGTCGCC GGAGCGGTCG AGTTCTGGAC CGACCGGCTC GGGTTCTCCC
     9181 GGGACTTCGT GGAGGACGAC TTCGCCGGTG TGGTCCGGGA CGACGTGACC CTGTTCATCA
     9241 GCGCGGTCCA GGACCAGGTG GTGCCGGACA ACACCCTGGC CTGGGTGTGG GTGCGCGGCC
     9301 TGGACGAGCT GTACGCCGAG TGGTCGGAGG TCGTGTCCAC GAACTTCCGG GACGCCTCCG
     9361 GGCCGGCCAT GACCGAGATC GGCGAGCAGC CGTGGGGGCG GGAGTTCGCC CTGCGCGACC
     9421 CGGCCGGCAA CTGCGTGCAC TTCGTGGCCG AGGAGCAGGA CTGACACGTG CTAAAACTTC
     9481 ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC
     9541 CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT
     9601 CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC
     9661 CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT
     9721 TCAGCAGAGC GCAGATACCA AATACTGTTC TTCTAGTGTA GCCGTAGTTA GGCCACCACT
     9781 TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG
     9841 CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA
     9901 AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA
     9961 CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG
    10021 GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG
    10081 AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC
    10141 TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA
    10201 ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG
    10261 CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC
    10321 GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA G
//
